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An initial denaturation was carried out for 45 s at 94 Β° C, 24 cycles of denaturation for 15 s at 94 Β° C, annealing-extension at 68 Β° C for 12 min, and a final extension at 72 Β° C for 10 min using the following primers: 5β²-CACTATCAACATATTCATCTCTTGTTTC-3β² (forward) and 5β²-GCTCTATGGACACCAATAGTTCCAAGGG-3β² (reverse)
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