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After two washes with PBS buffer, cells were lysed using glass beads and Mini-Beadbeater-16 (BioSpec Products) in ChIP lysis buffer [50 mM HEPES pH 7.5, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1 mM PMSF, and EDTA-free Protease Inhibitor Cocktail (Roche)]
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