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Since the diversity of generated hAbs depends on the successful introduction of the human Ig genome into mice, yeast artificial chromosome (YAC) vectors were utilized to introduce large segments of the human IgH and Igκ genomes into mouse embryonic stem (ES) cells through yeast spheroplast-ES cell fusion [4, 111, 112]

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Cell type deconvolution was performed using Robust Cell Type Decomposition (RCTD), with scRNA-seq data serving as the reference