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The A-side buffer contained 100 µM Lucifer yellow dye, in transport buffer (1.98 g/L glucose in 10 mM HEPES, 1× Hank’s balanced salt solution, pH 7.4), and the B-side buffer was transport buffer at pH 7.4

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Oxidative stress, mitochondrial-endothelial dysfunction, and insulin resistance are very common in cardiovascular diseases such as stroke, silent myocardial ischemia, coronary artery disease, or hypertension [390]